Device

Part:BBa_K933003

Designed by: Chris Cetnar   Group: iGEM12_Penn_State   (2012-10-11)


Bba_J23012 in directionality analysis construct

This part was used as the control for measuring directionality of promoter sequences. The specific part has the directionality construct with Bba_J23102 as the inserted promoter. By digesting at the restriction site xba1 and Spe1 this will remove the promoter sequence, one can then ligate in different promoters at the restriction sites to measure the directionality of different promoter sequences.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None